Isolation and identification of a new bifidogenic growth stimulator produced by Propionibacterium freudenreichii ET-3

Biosci Biotechnol Biochem. 2002 Mar;66(3):679-81. doi: 10.1271/bbb.66.679.


We have found a new growth stimulator for bifidobacteria in the culture broth of Propionibacterium freudenreichii ET-3. The bifidogenic growth stimulator (BGS) was purified by Diaion HP-20 column chromatography and preparative HPLC. Spectroscopic methods including 1H-NMR, UV, and LC-ESI-MS experiments indicated that the chemical structure of the bifidogenic growth stimulator was 1,4-dihydroxy-2-naphthoic acid (DHNA). Approximately 10 mg/L of DHNA was found to be produced in the culture broth of P. freudenreichii ET-3.

MeSH terms

  • Bifidobacterium / drug effects*
  • Bifidobacterium / growth & development*
  • Chromatography, High Pressure Liquid
  • Fermentation
  • Magnetic Resonance Spectroscopy
  • Naphthols / metabolism*
  • Naphthols / pharmacology*
  • Propionibacterium / metabolism*
  • Spectrophotometry, Ultraviolet


  • Naphthols
  • 1,4-dihydroxy-2-naphthoic acid