Quantitative trait locus mapping of genes that regulate HDL cholesterol in SM/J and NZB/B1NJ inbred mice

Physiol Genomics. 2002;9(2):93-102. doi: 10.1152/physiolgenomics.00107.2001.


To investigate the quantitative trait loci (QTL) regulating plasma cholesterol, the female progeny of an (SMxNZB/ B1NJ)xNZB/B1NJ backcross were fed an atherogenic diet. After 18 wk, plasma total cholesterol and high-density lipoprotein cholesterol (HDL-C) was measured. HDL-C concentrations were greater in NZB than in SM mice. For standard chow-fed mice, QTL were found near D5Mit370 and D18Mit34. For mice fed an atherogenic diet, a QTL was found near D5Mit239. The QTL for chow-fed and atherogenic-fed mice on chromosome 5 seem to be two different loci. We used a multitrait analysis to rule out pleiotropy in favor of a two-QTL hypothesis. Furthermore, the HDL-C in these strains was induced by the high-fat diet. For inducible HDL-C, one significant locus was found near D15Mit39. The gene for an HDL receptor, Srb1, maps close to the HDL-C QTL at D5Mit370, but the concentrations of Srb1 mRNA and SR-B1 protein and the gene sequence of NZB/B1NJ and SM/J did not support Srb1 as a candidate gene. With these QTL, we have identified chromosomal regions that affect lipoprotein profiles in these strains.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Carrier Proteins*
  • Cholesterol, HDL / blood*
  • Cholesterol, HDL / chemistry
  • Chromosome Mapping*
  • Crosses, Genetic
  • DNA, Complementary
  • Diet, Atherogenic
  • Female
  • Genotype
  • Lipoproteins, HDL*
  • Liver / metabolism
  • Male
  • Membrane Proteins*
  • Mice
  • Mice, Inbred NZB
  • Mice, Inbred Strains
  • Particle Size
  • Quantitative Trait, Heritable*
  • RNA-Binding Proteins*
  • Receptors, Lipoprotein / genetics
  • Receptors, Lipoprotein / metabolism
  • Scavenger Receptors, Class B


  • Carrier Proteins
  • Cholesterol, HDL
  • DNA, Complementary
  • Lipoproteins, HDL
  • Membrane Proteins
  • RNA-Binding Proteins
  • Receptors, Lipoprotein
  • Scarb1 protein, mouse
  • Scavenger Receptors, Class B
  • high density lipoprotein receptors
  • high density lipoprotein binding protein