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. 2002 Apr 19;109(2):193-203.
doi: 10.1016/s0092-8674(02)00724-9.

The Transcriptional Regulator RfaH Stimulates RNA Chain Synthesis After Recruitment to Elongation Complexes by the Exposed Nontemplate DNA Strand

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The Transcriptional Regulator RfaH Stimulates RNA Chain Synthesis After Recruitment to Elongation Complexes by the Exposed Nontemplate DNA Strand

Irina Artsimovitch et al. Cell. .

Erratum in

  • Cell 2002 Sep 20;110(6):801

Abstract

The transcriptional regulatory protein RfaH controls expression of several operons that encode extracytoplasmic components in bacteria. Regulation by RfaH occurs during transcript elongation and depends on a 5'-proximal, transcribed nucleic acid sequence called ops that induces transcriptional pausing in vitro and in vivo. We report that RfaH recognizes RNA polymerase transcribing RfaH-regulated operons by interacting with the ops sequence in the exposed nontemplate DNA strand of ops-paused transcription complexes. Although RfaH delays escape from the ops pause, once escape occurs, RfaH enhances elongation by suppressing pausing and rho-dependent termination without apparent involvement of other accessory proteins. This activity predicts a cumulative antitermination model for RfaH's regulation of ops-containing operons in vivo.

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