Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome

Trends Biotechnol. 2002 Jun;20(6):261-8. doi: 10.1016/s0167-7799(02)01944-3.


In signal transduction in eukaryotes, protein phosphorylation is a key event. To understand signaling processes, we must first acquire an inventory of phosphoproteins and their phosphorylation sites under different conditions. Because phosphorylation is a dynamic process, elucidation of signaling networks also requires quantitation of these phosphorylation events. In this article, we outline several methods for enrichment of phosphorylated proteins and peptides and discuss various options for their identification and quantitation with special emphasis on mass spectrometry-based techniques.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Antibodies / immunology
  • Chromatography / methods
  • Fourier Analysis
  • Mass Spectrometry / methods*
  • Phosphoproteins / analysis*
  • Phosphoproteins / chemistry
  • Phosphoproteins / immunology
  • Phosphorylation
  • Proteome / chemistry*
  • Signal Transduction


  • Antibodies
  • Phosphoproteins
  • Proteome