T type 1/type 2 subsets balance in B-cell chronic lymphocytic leukemia--the three-color flow cytometry analysis

Leuk Res. 2002 Jul;26(7):657-60. doi: 10.1016/s0145-2126(01)00194-1.


The impairments in immune cell functions as well as changes in cytokine network are the cause of malignant cell accumulation and secondary immune deficiencies in B-cell chronic lymphocytic leukemia (B-CLL). To broaden the knowledge of immune system in B-CLL we tried to evaluate the Th1/Th2 and Tc1/Tc2 balance in B-CLL patients in comparison with healthy individuals and changes of this pattern during disease progression. The three-color flow cytometry technique was used to analyze IL-4 and IFNgamma expression in peripheral blood CD3+CD4+ and CD3+CD8+ cells. The results indicate the dominance of T type 1 cells and T-cell-mediated immunity in B-CLL patients that is however shifted towards T type 2 during disease progression.

Publication types

  • Comparative Study

MeSH terms

  • Biomarkers
  • CD3 Complex / blood
  • CD4 Lymphocyte Count* / instrumentation
  • CD4 Lymphocyte Count* / methods
  • CD8 Antigens / blood
  • CD8-Positive T-Lymphocytes / chemistry
  • Disease Progression
  • Flow Cytometry / methods*
  • Humans
  • Immunity, Cellular
  • Interferon-gamma / blood
  • Interleukin-4 / blood
  • Leukemia, Lymphocytic, Chronic, B-Cell / immunology*
  • Th1 Cells* / chemistry
  • Th2 Cells* / chemistry


  • Biomarkers
  • CD3 Complex
  • CD8 Antigens
  • Interleukin-4
  • Interferon-gamma