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. 2002 May 15;28(3-4):549-57.
doi: 10.1016/s0731-7085(01)00687-2.

Validation of a LC-fluorescence Method for Determination of Free Captopril in Human Plasma, Using a Pre-Column Derivatization Reaction With Monobromobimane

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Validation of a LC-fluorescence Method for Determination of Free Captopril in Human Plasma, Using a Pre-Column Derivatization Reaction With Monobromobimane

Florentin Tache et al. J Pharm Biomed Anal. .

Abstract

Both derivatization of free captopril in human plasma samples using monobromobimane as fluorescent label and the corresponding HPLC-fluorescence detection (FLD) method were validated. Calibration curve for the fluorescent captopril derivative in plasma samples is linear in the ppb-ppm range with a detection limit of 4 ppb and an identification limit of 10 ppb (P%: 90; nu > or = 5). These methods were successfully applied on bioequivalence studies carried out on some marketed pharmaceutical formulations.

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