Determination of 11-keto-boswellic acid in human plasma

J Pharm Biomed Anal. 2002 May 15;28(3-4):729-39. doi: 10.1016/s0731-7085(01)00674-4.

Abstract

A sensitive, specific, accurate, fast and reproducible GC/MS-method for the quantitative determination of 11-keto-boswellic acid in human plasma using 18alpha-glycyrrhetinic acid as the internal standard was developed and validated. 11-Keto-boswellic acid and the internal standard were separated from acidified plasma by liquid/liquid extraction. The extracted samples were methylated and analyzed by GC/MS in the negative ion chemical ionization mode (NICI) and selected ion monitoring (SIM). The total run time was 8 min between injections. The assay described in this paper demonstrates a validated lower limit of quantification of 0.0100 microg/ml using 1 ml of plasma. The calibration curves are linear in the measured range between 10.0 and 2000 ng/ml plasma. The overall precision (expressed as CV) and accuracy (expressed as bias) for all concentrations of quality controls and standards is better than 15%. No indications were found for possible instabilities of 11-keto-boswellic acid in plasma, in whole blood, in the extraction solvent or after repeated thawing/freezing cycles. The recovery of the extraction method is calculated as 84%. The assay was applied successfully to determine the plasma level of 11-keto-boswellic acid in a clinical pilot study.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Biological Availability
  • Calibration
  • Chromatography, Gas
  • Dealkylation
  • Freezing
  • Indicators and Reagents
  • Mass Spectrometry
  • Medicine, Ayurvedic
  • Quality Control
  • Reference Standards
  • Reproducibility of Results
  • Solutions
  • Solvents
  • Triterpenes / blood*
  • Triterpenes / pharmacokinetics

Substances

  • 11-keto-boswellic acid
  • Indicators and Reagents
  • Solutions
  • Solvents
  • Triterpenes