Isolation and characterization of a novel human gene, VANGL1, as a therapeutic target for hepatocellular carcinoma

Int J Oncol. 2002 Jun;20(6):1173-8.


Through analysis of genome-wide expression profiles, we have been attempting to isolate novel molecular targets for diagnosis, treatment and prevention of hepatocellular carcinoma (HCC). Among the genes that a cDNA microarray showed to be commonly transactivated in HCCs, we identified a novel human homologue of the Drosophila Van Gogh/Strabismus gene and termed it VANGL1 [Vang (Van Gogh, Drosophila)-like 1]. This gene, which encoded a transcript with an open reading frame of 1,572 bp, was expressed specifically in testis and ovary among 16 adult normal tissues examined. Transfection of antisense S-oligonucleotides, but not of sense S-oligonucleotides, into hepatoma cells suppressed expression of VANGL1 and subsequent growth of these cells was inhibited to a marked degree. The findings suggest that VANGL1 might serve as a diagnostic marker for HCC as well as a potential molecular target for development of novel therapeutic drugs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Biomarkers, Tumor
  • COS Cells
  • Carcinoma, Hepatocellular / drug therapy
  • Carcinoma, Hepatocellular / genetics*
  • Carcinoma, Hepatocellular / pathology
  • Humans
  • Liver Neoplasms / drug therapy
  • Liver Neoplasms / genetics*
  • Liver Neoplasms / pathology
  • Mice
  • Molecular Sequence Data
  • Neoplasm Proteins / chemistry
  • Neoplasm Proteins / genetics*
  • Oligonucleotides, Antisense / therapeutic use


  • Biomarkers, Tumor
  • Neoplasm Proteins
  • Oligonucleotides, Antisense