In vivo imaging reveals different cellular functions for FGF and Dpp signaling in tracheal branching morphogenesis

Dev Cell. 2002 May;2(5):677-83. doi: 10.1016/s1534-5807(02)00171-5.

Abstract

In the developing tracheal system of Drosophila melanogaster, six major branches arise by guided cell migration from a sac-like structure. The chemoattractant Branchless/FGF (Bnl) appears to guide cell migration and is essential for the formation of all tracheal branches, while Decapentaplegic (Dpp) signaling is strictly required for the formation of a subset of branches, the dorsal and ventral branches. Using in vivo confocal video microscopy, we find that the two signaling systems affect different cellular functions required for branching morphogenesis. Bnl/FGF signaling affects the formation of dynamic filopodia, possibly controlling cytoskeletal activity and motility as such, and Dpp controls cellular functions allowing branch morphogenesis and outgrowth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Drosophila Proteins / genetics
  • Drosophila Proteins / physiology*
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / growth & development*
  • Drosophila melanogaster / physiology
  • Fibroblast Growth Factors / genetics
  • Fibroblast Growth Factors / physiology*
  • Genes, Insect
  • Insect Proteins / genetics
  • Insect Proteins / physiology
  • Morphogenesis
  • Pseudopodia / physiology
  • Signal Transduction
  • Trachea / growth & development*

Substances

  • Drosophila Proteins
  • Insect Proteins
  • bnl protein, Drosophila
  • dpp protein, Drosophila
  • Fibroblast Growth Factors