Coordinated Transcription of Key Pathways in the Mouse by the Circadian Clock

Cell. 2002 May 3;109(3):307-20. doi: 10.1016/s0092-8674(02)00722-5.

Abstract

In mammals, circadian control of physiology and behavior is driven by a master pacemaker located in the suprachiasmatic nuclei (SCN) of the hypothalamus. We have used gene expression profiling to identify cycling transcripts in the SCN and in the liver. Our analysis revealed approximately 650 cycling transcripts and showed that the majority of these were specific to either the SCN or the liver. Genetic and genomic analysis suggests that a relatively small number of output genes are directly regulated by core oscillator components. Major processes regulated by the SCN and liver were found to be under circadian regulation. Importantly, rate-limiting steps in these various pathways were key sites of circadian control, highlighting the fundamental role that circadian clocks play in cellular and organismal physiology.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Biological Clocks / genetics
  • Biological Clocks / physiology*
  • CLOCK Proteins
  • Cell Cycle / physiology
  • Circadian Rhythm / genetics
  • Circadian Rhythm / physiology*
  • Gene Expression Profiling
  • In Situ Hybridization
  • Liver / physiology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Mutation
  • Suprachiasmatic Nucleus / physiology
  • Trans-Activators / genetics
  • Trans-Activators / physiology
  • Transcription Factors / physiology
  • Transcription, Genetic / physiology*

Substances

  • Trans-Activators
  • Transcription Factors
  • CLOCK Proteins
  • Clock protein, mouse