Interferon-beta treatment alters peripheral blood monocytes chemokine production in MS patients

J Neuroimmunol. 2002 May;126(1-2):205-12. doi: 10.1016/s0165-5728(02)00064-4.


Chemokines direct the recruitment of leukocytes to inflammatory sites and may also participate in the regulation of cytokine production by naive T helper cells. Chemokine production by blood monocytes was investigated by intracytoplasmic staining in interferon-beta (IFN-beta)-treated multiple sclerosis (MS) patients, untreated MS patients, and healthy controls. Under unstimulated conditions, no differences in the production of interleukin-8 (IL-8), IFN-inducible protein 10 (IP-10), monokine induced by interferon-gamma (Mig), monocyte chemoattractant protein-1 (MCP-1), and monocyte chemoattractant protein-3 (MCP-3) were seen between untreated MS patients and controls. Chemokine production by monocytes following T cell activation was decreased in MS patients taking IFN-beta compared to controls and untreated MS patients. Unlike other chemokines, macrophage inflammatory protein-1alpha (MIP-1alpha) production by monocytes was significantly decreased in untreated MS patients compared to controls, and IFN-beta treatment increased MIP-1alpha expression to the level seen in controls. In vitro addition of IFN-beta1b to peripheral blood mononuclear cells (PBMC) cultures tended to decrease the production of IL-8, IP-10, Mig, MCP-1, and MCP-3, but not of MIP-1alpha. These findings suggest that IFN-beta treatment may have a differential affect on chemokine production by monocytes. Longitudinal studies must be done to confirm these observations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / administration & dosage*
  • Adult
  • CD40 Ligand / immunology
  • Chemokine CCL2 / biosynthesis
  • Chemokine CCL7
  • Chemokine CXCL10 / biosynthesis
  • Chemokine CXCL9
  • Chemokines, CXC / biosynthesis
  • Cytokines / biosynthesis*
  • Female
  • Humans
  • In Vitro Techniques
  • Intercellular Signaling Peptides and Proteins*
  • Interferon-beta / administration & dosage*
  • Interleukin-10 / immunology
  • Interleukin-8 / biosynthesis
  • Male
  • Monocyte Chemoattractant Proteins / biosynthesis
  • Monocytes / drug effects*
  • Monocytes / immunology
  • Monocytes / metabolism
  • Multiple Sclerosis / drug therapy*
  • Multiple Sclerosis / immunology
  • T-Lymphocytes / immunology


  • Adjuvants, Immunologic
  • CCL7 protein, human
  • CXCL9 protein, human
  • Chemokine CCL2
  • Chemokine CCL7
  • Chemokine CXCL10
  • Chemokine CXCL9
  • Chemokines, CXC
  • Cytokines
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-8
  • Monocyte Chemoattractant Proteins
  • Interleukin-10
  • CD40 Ligand
  • Interferon-beta