Abstract
We have identified the cuiD gene in Salmonella enterica serova Typhimurium that codes for a putative multicopper oxidase. Expression of cuiD is induced by copper ion and its promoter/operator has sequence similarity to the promoters controlled by the transcriptional regulators of the MerR family. We also identified and isolated a gene from S. enterica serova Typhimurium that encodes a 138-amino acid residue protein, sctR, a new member of the MerR family of transcriptional regulators. Transposon-insertional disruption of sctR shows sensitivity to copper ion and no response of cuiD expression. Copper-responsive induction and copper tolerance were restored by providing sctR in trans, suggesting that SctR plays an important role in intracellular copper detoxification.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Copper / pharmacology*
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DNA-Binding Proteins / genetics*
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Gene Expression Regulation, Bacterial / drug effects*
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Genetic Complementation Test
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Molecular Sequence Data
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Operon
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Oxidoreductases / chemistry
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Oxidoreductases / genetics*
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Promoter Regions, Genetic
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Salmonella typhimurium / drug effects
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Salmonella typhimurium / genetics*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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beta-Galactosidase / drug effects
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beta-Galactosidase / genetics
Substances
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Bacterial Proteins
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DNA-Binding Proteins
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MerR protein, Bacteria
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SctR protein, Salmonella typhimurium
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Transcription Factors
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Copper
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CiuD protein, Salmonella typhimurium
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Oxidoreductases
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copper oxidase
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beta-Galactosidase