Proton-activated fluorescence as a tool for simultaneous screening of combinatorial chemical reactions

Curr Opin Chem Biol. 2002 Jun;6(3):333-8. doi: 10.1016/s1367-5931(02)00324-1.


In recent years, combinatorial chemistry has had a significant impact on catalyst discovery in diverse fields. Proton-activated fluorescence (PAF) has been successfully demonstrated as a technique for effective screening of catalysts for electro-oxidation, enzymatic ester hydrolysis and nonenzymatic acyl transfer reactions. Among the working prototypes are screens for high-throughput assays of arrayed solid-state catalysts, dissolved enzymatic and small-molecule catalysts, as well as catalysts immobilized in solid-phase synthesis beads or polymeric gels. Given the range of reactions that may be set up to provide a change in local pH, the potential of PAF to facilitate catalyst discovery and process development is significant.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Catalysis
  • Combinatorial Chemistry Techniques / methods*
  • Fluorescence
  • Molecular Conformation
  • Protons


  • Protons