Changes in collagen matrix composition in human posterior tibial tendon dysfunction

Joint Bone Spine. 2002 Mar;69(2):189-94. doi: 10.1016/s1297-319x(02)00369-x.


Objective: To investigate whether tendon degeneration in posterior tibial tendon dysfunction syndrome is associated with changes in extracellular matrix collagen composition.

Methods: Specimens from grossly abnormal tendon regions from 9 patients with posterior tibial tendon dysfunction syndrome were prepared for routine histology. Collagens I, III and V were typed by immunoblotting and quantified by densitometry after SDS-PAGE. Proline and hydroxyproline residues were determined by liquid chromatography. Four other samples from grossly normal homologous tendon regions and one surgical specimen from a healthy patient undergoing arthrodesis of the ankle after an accident were included as control.

Results: In the grossly abnormal surgical posterior tibial tendon specimens we observed three types of histopathologic conditions present to varying degrees: increased mucin content, fibroblast hypercellularity and neovascularization. Analysis of degenerate tendons demonstrated a 79.3% increase in total proline and a 32.4% increase in 4-hydroxyproline. In addition, damaged tissue contained a higher proportion of collagen type III (mean increase: 53.6%) associated with a concomitant increase in type V collagen (mean increase: 26.4%). These alterations were accompanied by a reduction in type I collagen (mean decrease: 41.4%).

Conclusions: In posterior tibial tendon dysfunction syndrome, the degenerative process results from marked changes in both structural organization and molecular composition of matrix collagens. The higher proportion of type V and type IlI collagens in degenerated tendons is likely to contribute to a decrease in the mechanical resistance of the tissue.

MeSH terms

  • Aged
  • Chromatography, High Pressure Liquid
  • Collagen / classification
  • Collagen / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Extracellular Matrix / metabolism*
  • Female
  • Humans
  • Hydroxyproline / metabolism
  • Middle Aged
  • Posterior Tibial Tendon Dysfunction / complications
  • Posterior Tibial Tendon Dysfunction / metabolism*
  • Posterior Tibial Tendon Dysfunction / pathology
  • Proline / metabolism
  • Tendinopathy / etiology
  • Tendinopathy / metabolism
  • Tendinopathy / pathology
  • Tendons / metabolism
  • Tendons / pathology


  • Collagen
  • Proline
  • Hydroxyproline