CD80 expression is induced on activated B cells following stimulation by CD86

Scand J Immunol. 2002 Jun;55(6):577-84. doi: 10.1046/j.1365-3083.2002.01093.x.

Abstract

We examined the effects of CD86 ligation on surface densities of CD80 on activated B cells. For this, splenic B cells where CD86 levels had been enhanced upon anti-immunoglobulin M (IgM) treatment were employed. Incubation of such CD86-expressing B cells with anti-CD86 monoclonal antibody (MoAb) resulted in a marked upregulation in cell surface levels of CD80. Such an enhancement in surface levels was also accompanied by a marked increase in CD80 mRNA, suggesting that the effects of CD86-triggering are exerted at the level of CD80 message. Furthermore, coculture of CD86-upregulated B cells with CD28-expressing fibroblasts also led to the enhancement of CD80 levels, which was not obtained upon incubation with cells expressing cytotoxic T-lymphocyte-associated molecule (CTLA)-4-Ig. Finally, we also demonstrate that CD86-induced CD80 is fully competent to function as a costimulatory molecule, as revealed by its ability to enhance cytokine secretion by allogeneic T cells. Thus, these results reveal the existence of another pathway that regulates the expression of CD80, at least on activated B cells, which could have important implications in directing the course of an immune response.

MeSH terms

  • 3T3 Cells
  • Abatacept
  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigens, CD / immunology*
  • Antigens, Differentiation / immunology
  • B-Lymphocytes / immunology*
  • B7-1 Antigen / analysis
  • B7-1 Antigen / biosynthesis*
  • B7-2 Antigen
  • CD28 Antigens / immunology
  • CTLA-4 Antigen
  • Coculture Techniques
  • Female
  • Flow Cytometry
  • Immunoconjugates*
  • Lymphocyte Activation / immunology
  • Membrane Glycoproteins / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Specific Pathogen-Free Organisms
  • Th1 Cells / immunology
  • Transfection
  • Up-Regulation

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Differentiation
  • B7-1 Antigen
  • B7-2 Antigen
  • CD28 Antigens
  • CTLA-4 Antigen
  • Cd86 protein, mouse
  • Ctla4 protein, mouse
  • Immunoconjugates
  • Membrane Glycoproteins
  • Abatacept