Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus

Plant J. 2002 May;30(4):415-29. doi: 10.1046/j.1365-313x.2002.01297.x.

Abstract

The tobacco N gene confers resistance to tobacco mosaic virus (TMV) and encodes a Toll-interleukin-1 receptor/nucleotide binding site/leucine-rich repeat (TIR-NBS-LRR) class protein. We have developed and used a tobacco rattle virus (TRV) based virus induced gene silencing (VIGS) system to investigate the role of tobacco candidate genes in the N-mediated signalling pathway. To accomplish this we generated transgenic Nicotiana benthamiana containing the tobacco N gene. The transgenic lines exhibit hypersensitive response (HR) to TMV and restrict virus spread to the inoculated site. This demonstrates that the tobacco N gene can confer resistance to TMV in heterologous N. benthamiana. We have used this line to study the role of tobacco Rar1-, EDS1-, and NPR1/NIM1- like genes in N-mediated resistance to TMV using a TRV based VIGS approach. Our VIGS analysis suggests that these genes are required for N function. EDS1-like gene requirement for the N function suggests that EDS1 could be a common component of bacterial, fungal and viral resistance signalling mediated by the TIR-NBS-LRR class of resistance proteins. Requirement of Rar1- like gene for N-mediated resistance to TMV and some powdery mildew resistance genes in barley provide the first example of converging points in the disease resistance signalling pathways mediated by TIR-NBS-LRR and CC-NBS-LRR proteins. The TRV based VIGS approach as described here to study N-mediated resistance signalling will be useful for the analysis of not only disease resistance signalling pathways but also of other signalling pathways in genetically intractable plant systems.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis Proteins*
  • Capsid / genetics
  • Capsid Proteins*
  • Carrier Proteins / genetics
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics
  • Gene Silencing
  • Green Fluorescent Proteins
  • Immunity, Innate / genetics
  • Intracellular Signaling Peptides and Proteins
  • Light
  • Luminescent Proteins / genetics
  • Molecular Sequence Data
  • Plant Diseases / genetics*
  • Plant Diseases / virology
  • Plant Proteins / genetics*
  • Plants, Genetically Modified
  • Sequence Homology, Amino Acid
  • Signal Transduction / genetics
  • Tobacco / genetics*
  • Tobacco / virology
  • Tobacco Mosaic Virus / growth & development*

Substances

  • Arabidopsis Proteins
  • Capsid Proteins
  • Carrier Proteins
  • DNA-Binding Proteins
  • EDS1 protein, Arabidopsis
  • Intracellular Signaling Peptides and Proteins
  • Luminescent Proteins
  • N protein, Nicotiana glutinosa
  • NPR1 protein, Arabidopsis
  • Plant Proteins
  • Rar1 protein, Hordeum vulgare
  • coat protein, Tobacco rattle virus
  • Green Fluorescent Proteins