MET3 promoter: a tightly regulated promoter and its application in construction of conditional lethal strain

Curr Microbiol. 2002 Jul;45(1):37-40. doi: 10.1007/s00284-001-0046-0.

Abstract

The beta-galactosidase activity driven by MET3 promoter was assayed in the absence of methionine and in the presence of different concentration of methionine in medium. To compare its activity with GAL1 promoter and the data reported by Mumburg about MET25 promoter, the MET3 promoter was a weak but tightly controlled promoter. Its successful application in the construction of methionine-sensitive tri-mutant ( cln1 Delta cln2 Delta pho85 Delta) demonstrated that the MET3 promoter is a useful promoter in construction of conditional lethal strain and heterologous expression in Saccharomyces cerevisiae.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular / methods
  • Cyclin-Dependent Kinases / genetics
  • Cyclins / genetics
  • Gene Expression Regulation, Bacterial
  • Genes, Reporter
  • Methionine / metabolism
  • Models, Molecular
  • Promoter Regions, Genetic*
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins / genetics
  • Sulfate Adenylyltransferase / genetics*
  • beta-Galactosidase / biosynthesis
  • beta-Galactosidase / genetics

Substances

  • CLN1 protein, S cerevisiae
  • CLN2 protein, S cerevisiae
  • Cyclins
  • Saccharomyces cerevisiae Proteins
  • Methionine
  • Cyclin-Dependent Kinases
  • PHO85 protein, S cerevisiae
  • Sulfate Adenylyltransferase
  • beta-Galactosidase