Reversible suppression of protein synthesis in concert with polysome disaggregation during anoxia exposure in Littorina littorea

Mol Cell Biochem. 2002 Mar;232(1-2):121-7. doi: 10.1023/a:1014811017753.


Many marine invertebrates can live without oxygen for long periods of time, a capacity that is facilitated by the ability to suppress metabolic rate in anoxia to a value that is typically less than 10% of the normal aerobic rate. The present study demonstrates that a reduction in the rate of protein synthesis is one factor in the overall anoxia-induced metabolic suppression in the marine snail, Littorina littorea. The rate of [3H]leucine incorporation into newly translated protein in hepatopancreas isolated from 48 h anoxic snails was determined to be 49% relative to normoxic controls. However, protein concentration in hepatopancreas did not change during anoxia, suggesting a coordinated suppression of net protein turnover. Analysis of hepatopancreas samples from snails exposed to 24-72 h anoxia showed a gradual disaggregation of polysomes into monosomes. A re-aggregation of monosomes into polysomes was observed after 3 h of aerobic recovery. Analysis of fractions from the ribosome profile using radiolabeled probe to detect alpha-tubulin transcripts confirmed a general decrease in protein translation during anoxia exposure (transcript association with polysomes decreased) with a reversal during aerobic recovery. Western blotting of hepatopancreas samples from normoxic, 24 h anoxic, and 1 h aerobic recovered snails demonstrated that eIF-2alpha is substantially phosphorylated during anoxia exposure and dephosphorylated during normoxia and aerobic recovery, suggesting a decrease in translation initiation during anoxia exposure. These results suggest that metabolic suppression during anoxia exposure in L. littorea involves a decrease in protein translation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Electrophoresis, Gel, Two-Dimensional
  • Eukaryotic Initiation Factor-2 / analysis
  • Hypoxia / metabolism*
  • Mollusca / chemistry
  • Mollusca / drug effects
  • Mollusca / metabolism*
  • Oxygen / pharmacology
  • Polyribosomes / drug effects
  • Polyribosomes / metabolism*
  • Protein Biosynthesis* / drug effects
  • Proteins / analysis
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Time Factors
  • Tubulin / biosynthesis
  • Tubulin / genetics


  • Eukaryotic Initiation Factor-2
  • Proteins
  • RNA, Messenger
  • Tubulin
  • Oxygen