Abstract
In this study, we used the gastric epithelial cell line AGS-G(R) to investigate the role of GATA transcription factors in the regulation of both basal and gastrin-stimulated L-histidine decarboxylase (HDC) gene transcription. Using reporter gene technology, we compared the transcriptional activity of a construct, hHDC503, which contained the 5'-flanking region of the human HDC gene with that of similar constructs lacking selected GATA consensus sequences. We demonstrated the expression of GATA-4 and GATA-6 proteins within the AGS-G(R) cells and found evidence that these transcription factors can negatively regulate HDC gene expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line
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Consensus Sequence
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Electrophoretic Mobility Shift Assay
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Epithelial Cells / drug effects
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Epithelial Cells / enzymology
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Epithelial Cells / metabolism*
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GATA4 Transcription Factor
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GATA6 Transcription Factor
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Gastrins / antagonists & inhibitors
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Gastrins / pharmacology
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Gene Expression Regulation / drug effects
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Genes, Reporter
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Histidine Decarboxylase / biosynthesis*
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Humans
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Transcription Factors / genetics
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Transcription Factors / metabolism*
Substances
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DNA-Binding Proteins
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GATA4 Transcription Factor
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GATA6 Transcription Factor
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GATA6 protein, human
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Gastrins
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Transcription Factors
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Histidine Decarboxylase