GATA proteins are potential negative regulators of HDC gene expression in the gastric epithelium

Biochim Biophys Acta. 2002 Jun 7;1576(1-2):198-202. doi: 10.1016/s0167-4781(02)00301-9.

Abstract

In this study, we used the gastric epithelial cell line AGS-G(R) to investigate the role of GATA transcription factors in the regulation of both basal and gastrin-stimulated L-histidine decarboxylase (HDC) gene transcription. Using reporter gene technology, we compared the transcriptional activity of a construct, hHDC503, which contained the 5'-flanking region of the human HDC gene with that of similar constructs lacking selected GATA consensus sequences. We demonstrated the expression of GATA-4 and GATA-6 proteins within the AGS-G(R) cells and found evidence that these transcription factors can negatively regulate HDC gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Consensus Sequence
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Electrophoretic Mobility Shift Assay
  • Epithelial Cells / drug effects
  • Epithelial Cells / enzymology
  • Epithelial Cells / metabolism*
  • GATA4 Transcription Factor
  • GATA6 Transcription Factor
  • Gastrins / antagonists & inhibitors
  • Gastrins / pharmacology
  • Gene Expression Regulation / drug effects
  • Genes, Reporter
  • Histidine Decarboxylase / biosynthesis*
  • Humans
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*

Substances

  • DNA-Binding Proteins
  • GATA4 Transcription Factor
  • GATA6 Transcription Factor
  • GATA6 protein, human
  • Gastrins
  • Transcription Factors
  • Histidine Decarboxylase