Photochemical control of the infectivity of adenoviral vectors using a novel photocleavable biotinylation reagent

Chem Biol. 2002 May;9(5):567-73. doi: 10.1016/s1074-5521(02)00135-7.


We have explored a novel strategy for controlling the infectivity of adenoviral vectors. This strategy involves a method whereby the infectivity of adenoviral vectors is neutralized by treatment of viral particles with a water-soluble, photocleavable biotinylation reagent. These modified viral vectors possess little to no infectivity for target cells. Exposure of these modified viral vectors to 365 nm light induces a reversal of the neutralizing, chemical modification, resulting in restoration of infectivity to the viral vectors. The light-directed transduction of target cells by photoactivatable adenoviral vectors was demonstrated successfully both in vitro and in vivo. This photochemical infectivity trigger possesses great potential, both as a research tool and as a novel tactic for the delivery of gene-transfer agents, since the infectivity of adenoviral vectors can be controlled externally in a versatile manner.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae / drug effects
  • Adenoviridae / pathogenicity*
  • Adenoviridae / radiation effects
  • Animals
  • Biotin / chemical synthesis
  • Biotin / chemistry*
  • Biotinylation / methods
  • Blotting, Western / methods
  • Cell Survival / radiation effects
  • Dogs
  • Gene Expression / genetics
  • Genetic Vectors / drug effects
  • Genetic Vectors / radiation effects*
  • Humans
  • Mice
  • Mice, Nude
  • Photochemistry / methods
  • Time Factors
  • Tumor Cells, Cultured
  • Ultraviolet Rays
  • Virulence / radiation effects*


  • Biotin