The meiotic recombination checkpoint is regulated by checkpoint rad+ genes in fission yeast

EMBO J. 2002 Jun 3;21(11):2807-18. doi: 10.1093/emboj/21.11.2807.

Abstract

During the course of meiotic prophase, intrinsic double-strand breaks (DSBs) must be repaired before the cell can engage in meiotic nuclear division. Here we investigate the mechanism that controls the meiotic progression in Schizosaccharomyces pombe that have accumulated excess meiotic DSBs. A meiotic recombination-defective mutant, meu13Delta, shows a delay in meiotic progression. This delay is dependent on rec12+, namely on DSB formation. Pulsed-field gel electrophoresis analysis revealed that meiotic DSB repair in meu13Delta was retarded. We also found that the delay in entering nuclear division was dependent on the checkpoint rad+, cds1+ and mek1+ (the meiotic paralog of Cds1/Chk2). This implies that these genes are involved in a checkpoint that provides time to repair DSBs. Consistently, the induction of an excess of extrinsic DSBs by ionizing radiation delayed meiotic progression in a rad17(+)-dependent manner. dmc1Delta also shows meiotic delay, however, this delay is independent of rec12+ and checkpoint rad+. We propose that checkpoint monitoring of the status of meiotic DSB repair exists in fission yeast and that defects other than DSB accumulation can cause delays in meiotic progression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Cell Cycle Proteins / genetics*
  • Cell Nucleus / metabolism
  • Checkpoint Kinase 2
  • DNA Damage
  • DNA Repair
  • DNA-Binding Proteins*
  • Endonucleases / genetics
  • Gamma Rays
  • Gene Expression Regulation, Fungal*
  • Meiosis
  • Mutation
  • Phosphorylation
  • Protein Kinases / genetics
  • Protein-Serine-Threonine Kinases*
  • Recombination, Genetic
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces pombe Proteins / genetics*
  • Time Factors

Substances

  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • Meu13 protein, S pombe
  • Schizosaccharomyces pombe Proteins
  • rec12 protein, S pombe
  • rad9 protein
  • Protein Kinases
  • Checkpoint Kinase 2
  • Cds1 protein, S pombe
  • Protein-Serine-Threonine Kinases
  • rad3 protein, S pombe
  • Endonucleases
  • rad1 protein, S pombe