Isolation, characterization, and localization of beta-tubulin genomic clones of three Drosophila montium subgroup species

Genome. 2002 Jun;45(3):604-7. doi: 10.1139/g02-020.

Abstract

Genomic libraries were constructed from three Drosophila species, namely Drosophila auraria, Drosophila serrata, and Drosophila kikkawai, belonging to the Drosophila montium subgroup of the Drosophila melanogaster species group. Clones containing beta-tubulin specific sequences were isolated, characterized by restriction endonuclease digestions and Southern hybridizations, and mapped by in situ hybridization on the polytene chromosomes of the species studied. The distribution of the beta-tubulin loci was found to be similar in D. montium species and D. melanogaster.

MeSH terms

  • Animals
  • Chromosome Mapping
  • Drosophila / genetics*
  • Genomic Library
  • Species Specificity
  • Tubulin / genetics*

Substances

  • Tubulin