[Estimation of the irreversible inhibition reaction rate constants and of the concentration of cholinesterase active sites under commensurable concentrations of enzyme and inhibitors]

Biokhimiia. 1975 May-Jun;40(3):521-5.
[Article in Russian]

Abstract

Kinetic analysis of the interaction of butyrylcholinesterase and the phosphoorganic inhibitor GT-161 [(C2H2O)2P(O)SC2H4+N(CH3)2C6H5-1-] is carried out. Short time incubations of an enzyme and an inhibitor (1-3 sec), even under commensurable concentrations, were shown to enable the rate constants of irreversible enzyme inhibition to be calcualted by the formula for pseudomonomoleuclar reactions. A simple analytical method for the estimation of the enzyme active sites concentrations is proposed.

Publication types

  • English Abstract

MeSH terms

  • Binding Sites
  • Cholinesterase Inhibitors* / pharmacology
  • Cholinesterases* / metabolism
  • Kinetics
  • Mathematics
  • Organothiophosphorus Compounds / pharmacology
  • Protein Binding
  • Time Factors

Substances

  • Cholinesterase Inhibitors
  • Organothiophosphorus Compounds
  • Cholinesterases