Interleukin-17 stimulates the expression of interleukin-8, growth-related oncogene-alpha, and granulocyte-colony-stimulating factor by human airway epithelial cells

Am J Respir Cell Mol Biol. 2002 Jun;26(6):748-53. doi: 10.1165/ajrcmb.26.6.4757.


Interleukin (IL)-17 is a recently discovered cytokine, which is proposed to play a role in neutrophilic airway inflammation via the release of proinflammatory cytokines and chemokines. To evaluate the role of IL-17 in inflammatory protein production from the airway epithelium, we have analyzed the effects of IL-17 on primary human bronchial epithelial cells (HBECs). Using gene arrays, changes in gene expression in response to IL-17 stimulation were investigated and only IL-8, growth-related oncogene (Gro)alpha, and granulocyte colony-stimulating factor (G-CSF) were found to be upregulated. Secretion of IL-8, Groalpha, and G-CSF in response to IL-17 was measured in HBEC cell culture supernatants by enzyme-linked immunosorbent assay. Upregulation of Groalpha, IL-8, and G-CSF was observed to be 8-, 5-, and 8-fold, respectively, after 48 h stimulation with IL-17. When tested at equivalent concentrations, IL-17 was found to be 2- to 3-fold more potent than tumor necrosis factor (TNF)-alpha in stimulating release of Groalpha and G-CSF from HBECs. In addition, IL-17 was found to synergistically enhance TNF-alpha-induced production of IL-8, Groalpha, and G-CSF. It is proposed that IL-17 may play an important role in neutrophil recruitment via stimulating the release of IL-8, Groalpha, and G-CSF from airway epithelial cells.

MeSH terms

  • Base Sequence
  • Bronchi / cytology
  • Bronchi / drug effects
  • Bronchi / metabolism*
  • DNA Primers
  • Dexamethasone / pharmacology
  • Enzyme-Linked Immunosorbent Assay
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism
  • Gene Expression Regulation / physiology*
  • Granulocyte Colony-Stimulating Factor / genetics*
  • Humans
  • Interleukin-17 / physiology*
  • Interleukin-8 / genetics*
  • Leupeptins / pharmacology
  • Oncogenes*
  • RNA, Messenger / genetics
  • Tumor Necrosis Factor-alpha / physiology


  • DNA Primers
  • Interleukin-17
  • Interleukin-8
  • Leupeptins
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • Granulocyte Colony-Stimulating Factor
  • Dexamethasone
  • benzyloxycarbonylleucyl-leucyl-leucine aldehyde