Autoxidation kinetic analysis of docosahexaenoic acid ethyl ester and docosahexaenoic triglyceride with oxygen sensor

Biosci Biotechnol Biochem. 2002 Apr;66(4):749-53. doi: 10.1271/bbb.66.749.


The application of omega-3 polyunsaturated fatty acids (PUFAs) as food additives is restricted by their chemically quite reactive properties. However, quantitative analyses of the oxidative kinetics of PUFAs are very few compared to other studies on food chemistry. In this study, the autoxidation kinetics of ethyl docosahexaenoate (DHAEE), docosahexaenoic triglyceride (DHA oil), and emulsified DHA oil were investigated with an oxygen sensor. The autocatalytic reaction rate constants for DHAEE, DHA oil, and the emulsified DHA oil with 20% (w/v) GA, 20% SSPS, or 20% SSPS containing 5% soy protein were obtained at 35, 50, and 70 degrees C. A plot of the natural logarithm of the frequency factor, In ka0, vs. the activation energy, Ea, demonstrated that In ka0 against Ea fitted well with a single straight line both for the data from this study and for other reported results. This implies that the chemical compensation relationship holds between ka0 and Ea for PUFA and emulsified DHA oil.

MeSH terms

  • Biosensing Techniques
  • Docosahexaenoic Acids / chemistry*
  • Emulsions
  • Indicators and Reagents
  • Kinetics
  • Oils / chemistry
  • Oxidation-Reduction
  • Oxygen / analysis*
  • Soybean Proteins
  • Triglycerides / chemistry*


  • Emulsions
  • Indicators and Reagents
  • Oils
  • Soybean Proteins
  • Triglycerides
  • Docosahexaenoic Acids
  • 4,7,10,13,16,19-docosahexaenoic acid ethyl ester
  • Oxygen