Abstract
Auxin-regulated gene expression is mediated by two families of transcription factors. The ARF proteins bind to a conserved DNA sequence called the AuxRE and activate transcription. The Aux/IAA proteins repress ARF function, presumably by forming dimers with ARF proteins. Recent genetic studies in Arabidopsis indicate that auxin regulates this system by promoting the ubiquitin-mediated degradation of the Aux/IAA proteins, thus permitting ARF function. Mutations in components of SCF(TIR1), a ubiquitin protein ligase (E3) result in stabilization of Aux/IAA proteins and decreased auxin response. Further, recent biochemical experiments indicate that the Aux/IAA proteins bind SCF(TIR1) in an auxin-dependent manner.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
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Review
MeSH terms
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Arabidopsis / drug effects
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Arabidopsis / genetics
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Arabidopsis / metabolism
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Cysteine Endopeptidases / metabolism
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Gene Expression Regulation, Plant / drug effects
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Indoleacetic Acids / metabolism
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Indoleacetic Acids / pharmacology*
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Models, Biological
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Multienzyme Complexes / metabolism
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Plant Proteins / metabolism*
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Plants / drug effects*
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Plants / genetics
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Plants / metabolism
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Proteasome Endopeptidase Complex
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Protein Binding
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Signal Transduction
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Transcription Factors / metabolism
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Ubiquitin / metabolism
Substances
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Indoleacetic Acids
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Multienzyme Complexes
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Plant Proteins
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Transcription Factors
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Ubiquitin
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Cysteine Endopeptidases
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Proteasome Endopeptidase Complex