Construction and characterization of an infectious molecular clone derived from the CRF01_AE primary isolate of HIV type 1

Tetsu Mukai; Satoshi Komoto; Takeshi Kurosu; J Alejandro Palacios; Yong-Gang Li; Wattana Auwanit; Masashi Tatsumi; Kazuyoshi Ikuta

doi:10.1089/088922202753747932

Construction and characterization of an infectious molecular clone derived from the CRF01_AE primary isolate of HIV type 1

AIDS Res Hum Retroviruses. 2002 May 20;18(8):585-9. doi: 10.1089/088922202753747932.

Authors

Tetsu Mukai¹, Satoshi Komoto, Takeshi Kurosu, J Alejandro Palacios, Yong-Gang Li, Wattana Auwanit, Masashi Tatsumi, Kazuyoshi Ikuta

Affiliation

¹ Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.

PMID: 12036488
DOI: 10.1089/088922202753747932

Abstract

An infectious molecular clone (named p95TNIH022) was constructed using long-range polymerase chain reaction products derived from a clinical isolate (95TNIH022) of HIV-1 CRF01_AE obtained from an asymptomatic Thai carrier in 1995. The virus in the supernatant from p95TNIH022-transfected 293T cells showed infectivity in peripheral blood mononuclear cells (PBMCs) as well as in MAGIC5 cells, which express CD4 and CCR5, but not in the original MAGI cells, indicating that p95TNIH022 is an infectious molecular clone with CCR5 tropism. Interestingly, p95TNIH022-derived virus induced profound cell killing in infected PBMCs, as in cells infected with the parental isolate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
CD4 Lymphocyte Count
Cell Line
Cloning, Molecular
DNA Primers
HIV Infections / virology*
HIV-1 / genetics
HIV-1 / pathogenicity*
Humans
Virulence*

Substances

DNA Primers