Deep lamellar keratoplasty with trypan blue intrastromal staining

J Cataract Refract Surg. 2002 Jun;28(6):929-31. doi: 10.1016/s0886-3350(02)01225-7.


This technique was developed to facilitate removal of the deep stromal layers during deep lamellar keratoplasty. A trypan blue 0.02% solution is injected into the stromal fibers, enabling the surgeon to visualize and remove the posterior stromal layers. This decreases the risk of perforation of Descemet's membrane and the endothelium.

MeSH terms

  • Coloring Agents*
  • Corneal Diseases / diagnosis
  • Corneal Diseases / surgery
  • Corneal Stroma / pathology*
  • Corneal Stroma / surgery*
  • Corneal Transplantation / methods*
  • Humans
  • Injections
  • Staining and Labeling / methods
  • Trypan Blue*


  • Coloring Agents
  • Trypan Blue