Comparison insight dual X-ray absorptiometry (DXA), histomorphometry, ash weight, and morphometric indices for bone evaluation in an animal model (the orchidectomized rat) of male osteoporosis

Calcif Tissue Int. 2001 Jan;68(1):31-7. doi: 10.1007/BF02685000. Epub 2000 Dec 22.


We have compared the measurements obtained by different methods: dual energy X-ray absorptiometry (DXA), histomorphometry, ash weight, and two morphometric indices (robusticity and bone weight/bone length index) in the orchidectomized (ORX) rat model of male osteoporosis. We examined 144 male wistar rats: 48 sham-operated, 48 ORX, and 48 ORX-treated with a bisphosphonate (risedronate) 2 or 10 mg/kg/day, 5 days per week. Rats were sacrificed at 2, 4, 8, or 16 weeks after the beginning of the study. DXA was performed on a Hologic QDR 2000 on the whole body, whole tibia, and tibial metaphysis. Bone volumes (C.BV/C.TV, and BV/TV) were measured by histomorphometry on the proximal tibial. A significant correlation was obtained between weight measured by DXA and scale (r = 0.993, P <0.000001). However, DXA underestimated weight by 0.3%. This discrepancy was dependent on the rat's weight. The weight bone length (WL) index was linearly correlated with BMD (r = 0.86), BMC (r = 0.96), and ash weight (r = 0.97). Correlation with robusticity was lower than with the WL index. A significant correlation was found between BMC of the metaphyseal region and the bone volumes but this explained only 27% of the variance; correlation with BMD was poorer (r = 0.40). BMC and ash weight were highly correlated (r = 0.992, P <0.000001). However, DXA overestimated BMC by 11% and the overestimation was found to be clearly dependent on the net mineral content of the bone.

Publication types

  • Comparative Study

MeSH terms

  • Absorptiometry, Photon
  • Animals
  • Body Weight / physiology
  • Bone Density / physiology*
  • Bone and Bones / diagnostic imaging
  • Bone and Bones / metabolism
  • Bone and Bones / physiopathology*
  • Climacteric / metabolism
  • Disease Models, Animal
  • Etidronic Acid / analogs & derivatives*
  • Etidronic Acid / pharmacology
  • Hypogonadism / complications*
  • Male
  • Orchiectomy / adverse effects
  • Organ Size / physiology*
  • Osteoporosis / diagnostic imaging
  • Osteoporosis / metabolism
  • Osteoporosis / physiopathology*
  • Predictive Value of Tests
  • Rats
  • Rats, Wistar
  • Risedronic Acid
  • Statistics as Topic
  • Tibia / diagnostic imaging
  • Tibia / metabolism
  • Tibia / physiopathology


  • Risedronic Acid
  • Etidronic Acid