Cancer/testis antigen CSAGE is concurrently expressed with MAGE in chondrosarcoma

Gene. 2002 Feb 20;285(1-2):269-78. doi: 10.1016/s0378-1119(02)00395-5.


Differential display-polymerase chain reaction was used to compare gene expression between human chondrosarcoma cell lines and normal cartilage. A new gene, CSAGE, has been cloned and belongs to a gene family that includes the taxol resistance associated gene (TRAG)-3. CSAGE, like TRAG-3, does not confer resistance to taxol when transfected in vitro. Both genes have alternatively spliced variants. CSAGE and TRAG-3 are expressed in chondrosarcoma, melanoma, and cartilage and testis, but not in other normal tissues. TRAG-3 has been reported to be a cancer/testis antigen. Our results suggest that CSAGE belongs to the growing list of cancer/testis antigens as well. In all of the CSAGE positive samples, the melanoma antigen gene family was also expressed. This is the first report on the expression of cancer/testis antigens in chondrosarcoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Antigens, Neoplasm / genetics*
  • Base Sequence
  • Blotting, Northern
  • Chondrosarcoma / genetics*
  • Chondrosarcoma / pathology
  • Drug Resistance, Neoplasm
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Humans
  • K562 Cells
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics*
  • Paclitaxel / pharmacology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Tumor Cells, Cultured / drug effects


  • Antigens, Neoplasm
  • CSAG1 protein, human
  • CSAG2 protein, human
  • Neoplasm Proteins
  • RNA, Messenger
  • Paclitaxel

Associated data

  • GENBANK/AF195880
  • GENBANK/AF268418
  • GENBANK/AF268419
  • GENBANK/AF268420
  • GENBANK/AF268421