Biodegradation of cis-dichloroethene as the sole carbon source by a beta-proteobacterium

Appl Environ Microbiol. 2002 Jun;68(6):2726-30. doi: 10.1128/aem.68.6.2726-2730.2002.

Abstract

An aerobic bacterium capable of growth on cis-dichloroethene (cDCE) as a sole carbon and energy source was isolated by enrichment culture. The 16S ribosomal DNA sequence of the isolate (strain JS666) had 97.9% identity to the sequence from Polaromonas vacuolata, indicating that the isolate was a beta-proteobacterium. At 20 degrees C, strain JS666 grew on cDCE with a minimum doubling time of 73 +/- 7 h and a growth yield of 6.1 g of protein/mol of cDCE. Chloride analysis indicated that complete dechlorination of cDCE occurred during growth. The half-velocity constant for cDCE transformation was 1.6 +/- 0.2 microM, and the maximum specific substrate utilization rate ranged from 12.6 to 16.8 nmol/min/mg of protein. Resting cells grown on cDCE could transform cDCE, ethene, vinyl chloride, trans-dichloroethene, trichloroethene, and 1,2-dichloroethane. Epoxyethane was produced from ethene by cDCE-grown cells, suggesting that an epoxidation reaction is the first step in cDCE degradation.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biodegradation, Environmental
  • Dichloroethylenes / metabolism*
  • Proteobacteria / classification
  • Proteobacteria / growth & development
  • Proteobacteria / metabolism*
  • Thermodynamics

Substances

  • Dichloroethylenes