siRNA-directed inhibition of HIV-1 infection

Nat Med. 2002 Jul;8(7):681-6. doi: 10.1038/nm725. Epub 2002 Jun 3.


RNA interference silences gene expression through short interfering 21 23-mer double-strand RNA segments that guide mRNA degradation in a sequence-specific fashion. Here we report that siRNAs inhibit virus production by targeting the mRNAs for either the HIV-1 cellular receptor CD4, the viral structural Gag protein or green fluorescence protein substituted for the Nef regulatory protein. siRNAs effectively inhibit pre- and/or post-integration infection events in the HIV-1 life cycle. Thus, siRNAs may have potential for therapeutic intervention in HIV-1 and other viral infections.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • CD4 Antigens / physiology
  • Cell Line
  • Gene Silencing*
  • HIV-1 / drug effects
  • HIV-1 / genetics
  • HIV-1 / physiology*
  • HeLa Cells
  • Humans
  • RNA, Antisense / pharmacology
  • RNA, Messenger / genetics
  • RNA, Small Interfering
  • RNA, Untranslated / pharmacology
  • RNA, Untranslated / physiology*
  • Receptors, CCR5 / physiology
  • Transfection
  • Virus Integration / drug effects


  • CD4 Antigens
  • RNA, Antisense
  • RNA, Messenger
  • RNA, Small Interfering
  • RNA, Untranslated
  • Receptors, CCR5