DNA replication in eukaryotic cells

Annu Rev Biochem. 2002;71:333-74. doi: 10.1146/annurev.biochem.71.110601.135425. Epub 2001 Nov 9.

Abstract

The maintenance of the eukaryotic genome requires precisely coordinated replication of the entire genome each time a cell divides. To achieve this coordination, eukaryotic cells use an ordered series of steps to form several key protein assemblies at origins of replication. Recent studies have identified many of the protein components of these complexes and the time during the cell cycle they assemble at the origin. Interestingly, despite distinct differences in origin structure, the identity and order of assembly of eukaryotic replication factors is highly conserved across all species. This review describes our current understanding of these events and how they are coordinated with cell cycle progression. We focus on bringing together the results from different organisms to provide a coherent model of the events of initiation. We emphasize recent progress in determining the function of the different replication factors once they have been assembled at the origin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Animals
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Cell Cycle / physiology*
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism
  • Chromatin / genetics
  • Chromatin / metabolism
  • Cyclin-Dependent Kinases / metabolism
  • DNA Replication / physiology*
  • DNA-Binding Proteins / metabolism
  • Eukaryotic Cells / metabolism*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Minichromosome Maintenance Proteins
  • Models, Genetic
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • Origin Recognition Complex
  • Saccharomyces cerevisiae Proteins*

Substances

  • CDC45 protein, S cerevisiae
  • CDC6 protein, S cerevisiae
  • Carrier Proteins
  • Cell Cycle Proteins
  • Chromatin
  • DNA-Binding Proteins
  • DPB11 protein, S cerevisiae
  • Fungal Proteins
  • MCM10 protein, human
  • Nuclear Proteins
  • Origin Recognition Complex
  • Saccharomyces cerevisiae Proteins
  • Adenosine Triphosphate
  • Cyclin-Dependent Kinases
  • Minichromosome Maintenance Proteins