Subtle mutations in the cysteine region of HIV-1 Vif drastically alter the viral replication phenotype

Microbes Infect. 2002 May;4(6):621-4. doi: 10.1016/s1286-4579(02)01580-0.


Mutations were introduced into the region encoding the two cysteine and nearby amino acid residues of human immunodeficiency virus type 1 (HIV-1) Vif protein and, 12 single-amino-acid viral mutants were constructed. Determination of their growth characteristics in two lymphocytic cell lines revealed that only a single amino acid change in the cysteine region greatly altered the replication phenotype. In particular, the four mutants of amino acid 132 of Vif were grouped into three categories on the basis of their growth potentials. These results indicate that the cysteine region of Vif is critical for the cell-dependent replication efficiency of HIV-1.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Cysteine / genetics
  • Cysteine / metabolism
  • Gene Products, vif / genetics*
  • Gene Products, vif / metabolism
  • HIV Reverse Transcriptase / analysis
  • HIV Reverse Transcriptase / biosynthesis
  • HIV-1 / genetics
  • HIV-1 / growth & development*
  • HIV-1 / metabolism
  • Humans
  • Mutagenesis, Site-Directed
  • Virus Replication / genetics
  • vif Gene Products, Human Immunodeficiency Virus


  • Gene Products, vif
  • vif Gene Products, Human Immunodeficiency Virus
  • HIV Reverse Transcriptase
  • Cysteine