The Sulfinator: predicting tyrosine sulfation sites in protein sequences

Bioinformatics. 2002 May;18(5):769-70. doi: 10.1093/bioinformatics/18.5.769.


Protein tyrosine sulfation is an important post-translational modification of proteins that go through the secretory pathway. No clear-cut acceptor motif can be defined that allows the prediction of tyrosine sulfation sites in polypeptide chains. The Sulfinator is a software tool that can be used to predict tyrosine sulfation sites in protein sequences with an overall accuracy of 98%. Four different Hidden Markov Models were constructed, each of them specialized to recognize sulfated tyrosine residues depending on their location within the sequence: near the N-terminus, near the C-terminus, in the center of a window with a size of at least 25 amino acids, as well as in windows containing several tyrosine residues.

Availability: The Sulfinator is accessible at (

Supplementary information: Sulfinator documentation is accessible at (

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / metabolism
  • Database Management Systems*
  • Databases, Protein
  • Drosophila / genetics
  • Drosophila / metabolism
  • Humans
  • Molecular Sequence Data
  • Proteins / genetics
  • Proteins / metabolism
  • Sensitivity and Specificity
  • Sequence Alignment
  • Sequence Analysis, Protein / methods*
  • Software*
  • Sulfates / metabolism*
  • Tyrosine / genetics
  • Tyrosine / metabolism*


  • Proteins
  • Sulfates
  • Tyrosine