A novel Xenopus Smad-interacting forkhead transcription factor (XFast-3) cooperates with XFast-1 in regulating gastrulation movements

Development. 2002 Jun;129(12):2823-34.

Abstract

In early Xenopus embryos, the prototypical XFast-1/Smad2/Smad4 complex ARF1 is induced at the Mix.2 ARE by activin overexpression. We have characterised ARF2, a related, but much more abundant, complex formed during gastrulation in response to endogenous TGFbeta family members and we have identified a novel Fast family member, XFast-3, as its transcription factor component. Endogenous ARF2 efficiently competes out ARF1 at early gastrulation, due to the ability of XFast-3 to interact with activated Smads with much higher affinity than XFast-1. We demonstrate that ARF1 and ARF2 are activated by distinct TGFbeta family members. Using morpholino antisense oligonucleotides to deplete levels of the constituent transcription factors XFast-1 and XFast-3 specifically, we demonstrate an important role for ARF1 and ARF2 in early Xenopus embryos in controlling the convergent extension movements of gastrulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • ADP-Ribosylation Factor 1 / metabolism
  • ADP-Ribosylation Factors / genetics
  • Amino Acid Sequence
  • Animals
  • Binding, Competitive
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Embryo, Nonmammalian
  • Female
  • Forkhead Transcription Factors
  • Gastrula*
  • Gene Expression Regulation, Developmental
  • Growth Substances / genetics
  • Growth Substances / metabolism
  • Intercellular Signaling Peptides and Proteins*
  • Mice
  • Molecular Sequence Data
  • Nerve Growth Factors
  • Oligonucleotides, Antisense / genetics
  • Response Elements
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Smad Proteins
  • Smad2 Protein
  • Smad4 Protein
  • T-Box Domain Proteins / genetics
  • T-Box Domain Proteins / metabolism
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism*
  • Xenopus / embryology*
  • Xenopus / metabolism
  • Xenopus Proteins / genetics*
  • Xenopus Proteins / metabolism

Substances

  • DNA-Binding Proteins
  • FOXH1 protein, Xenopus
  • Forkhead Transcription Factors
  • Foxh1 protein, mouse
  • GDF3 protein, Xenopus
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Nerve Growth Factors
  • Oligonucleotides, Antisense
  • Saccharomyces cerevisiae Proteins
  • Smad Proteins
  • Smad2 Protein
  • Smad2 protein, Xenopus
  • Smad2 protein, mouse
  • Smad4 Protein
  • Smad4 protein, mouse
  • T-Box Domain Proteins
  • Trans-Activators
  • Transcription Factors
  • VegT protein, Xenopus
  • Xenopus Proteins
  • foxh1.2 protein, Xenopus
  • smad4.1 protein, Xenopus
  • smad4.2 protein, Xenopus
  • ARF2 protein, S cerevisiae
  • Arf2 protein, mouse
  • ADP-Ribosylation Factor 1
  • ADP-Ribosylation Factors