A Conserved RNA-binding Protein Controls Germline Stem Cells in Caenorhabditis Elegans

Nature. 2002 Jun 6;417(6889):660-3. doi: 10.1038/nature754. Epub 2002 May 22.

Abstract

Germline stem cells are defined by their unique ability to generate more of themselves as well as differentiated gametes. The molecular mechanisms controlling the decision between self-renewal and differentiation are central unsolved problems in developmental biology with potentially broad medical implications. In Caenorhabditis elegans, germline stem cells are controlled by the somatic distal tip cell. FBF-1 and FBF-2, two nearly identical proteins, which together are called FBF ('fem-3 mRNA binding factor'), were originally discovered as regulators of germline sex determination. Here we report that FBF also controls germline stem cells: in an fbf-1 fbf-2 double mutant, germline proliferation is initially normal, but stem cells are not maintained. We suggest that FBF controls germline stem cells, at least in part, by repressing gld-1, which itself promotes commitment to the meiotic cell cycle. FBF belongs to the PUF family ('Pumilio and FBF') of RNA-binding proteins. Pumilio controls germline stem cells in Drosophila females, and, in lower eukaryotes, PUF proteins promote continued mitoses. We suggest that regulation by PUF proteins may be an ancient and widespread mechanism for control of stem cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3' Untranslated Regions / genetics
  • 3' Untranslated Regions / metabolism
  • Animals
  • Base Sequence
  • Caenorhabditis elegans / cytology*
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / growth & development
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism
  • Cell Differentiation
  • Cell Division
  • Cell Lineage
  • Conserved Sequence*
  • Disorders of Sex Development / genetics
  • Electrophoretic Mobility Shift Assay
  • Female
  • Gene Expression Regulation*
  • Genes, Helminth / genetics
  • Germ Cells / cytology*
  • Germ Cells / metabolism
  • Helminth Proteins / genetics
  • Helminth Proteins / metabolism*
  • Male
  • Meiosis
  • Mutation / genetics
  • Protein Binding
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism
  • Response Elements / genetics
  • Stem Cells / cytology*
  • Stem Cells / metabolism
  • Two-Hybrid System Techniques

Substances

  • 3' Untranslated Regions
  • Caenorhabditis elegans Proteins
  • Helminth Proteins
  • RNA-Binding Proteins
  • Repressor Proteins
  • fem-3-binding protein, C elegans