Skeletal changes in rats given daily subcutaneous injections of recombinant human parathyroid hormone (1-34) for 2 years and relevance to human safety

Toxicol Pathol. May-Jun 2002;30(3):312-21. doi: 10.1080/01926230252929882.

Abstract

Fischer 344 rats (60/sex/group) were given daily subcutaneous injections of recombinant human parathyroid hormone (PTH)(1-34) for 2 years at doses of 0, 5, 30, or 75 microg/kg. Treatment caused substantial increases in bone mass consistent with the known pharmacologic effects of once-daily administration. As determined by quantitative computed tomography (QCT) and histomorphometry, bone mass was markedly increased. Substantial new bone formation resulted in a large decrease in marrow space accompanied by altered bone architecture. Bone proliferative lesions were observed in all PTH( 1-34)-treated groups. Osteosarcoma occurred in 3, 21, and 31 male rats and in 4, 12, and 23 female rats in the 5-, 30-, and 75-microg/kg treatment groups, respectively. Focal osteoblast hyperplasia, osteoma, and osteoblastoma were much less frequent. Although the specific cellular or molecular mechanisms responsible for the rat bone tumors have not been fully elucidated, the data suggest that these lesions resulted from the long duration of treatment and the exaggerated pharmacologic response of the rat skeleton to daily treatment with PTH(1-34). Important differences between the rat study and clinical use in adult humans suggest that the increased incidence of bone neoplasia in rats treated for 2 years is likely not predictive of an increased risk of bone cancer in skeletally mature adult humans being given PTH(1-34) for a limited period of time in the treatment of osteoporosis.

MeSH terms

  • Animals
  • Bone Density / drug effects*
  • Bone Neoplasms / chemically induced*
  • Bone Neoplasms / pathology*
  • Drug Evaluation, Preclinical
  • Female
  • Humans
  • Hyperplasia / chemically induced
  • Injections, Subcutaneous
  • Male
  • Osteoblastoma / chemically induced
  • Osteoblastoma / pathology
  • Osteoma / chemically induced
  • Osteoma / pathology
  • Osteosarcoma / chemically induced
  • Osteosarcoma / pathology
  • Parathyroid Hormone / administration & dosage
  • Parathyroid Hormone / adverse effects*
  • Parathyroid Hormone / pharmacology*
  • Peptide Fragments / administration & dosage
  • Peptide Fragments / adverse effects*
  • Peptide Fragments / pharmacology*
  • Rats
  • Rats, Inbred F344
  • Recombinant Proteins / administration & dosage
  • Recombinant Proteins / adverse effects
  • Recombinant Proteins / pharmacology
  • Time Factors

Substances

  • Parathyroid Hormone
  • Peptide Fragments
  • Recombinant Proteins