Purification and partial gene sequence of the tyrosine decarboxylase of Lactobacillus brevis IOEB 9809

FEMS Microbiol Lett. 2002 May 21;211(1):85-9. doi: 10.1111/j.1574-6968.2002.tb11207.x.

Abstract

Some lactic acid bacteria contain a tyrosine decarboxylase (TDC) which converts tyrosine to tyramine, a biogenic amine frequently encountered in fermented food and wine. Purification and microsequencing of the TDC of Lactobacillus brevis IOEB 9809 allowed us to determine a partial sequence of the TDC gene encoding 264 amino acids of the enzyme. Analysis of this protein sequence revealed typical features of pyridoxal phosphate-dependent amino acid decarboxylases while not any known decarboxylase was closely related to the TDC of L. brevis IOEB 9809. In addition, we could detect other L. brevis strains carrying a TDC gene in a rapid assay based on the polymerase chain reaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Genes, Bacterial / genetics
  • Lactobacillus / enzymology*
  • Lactobacillus / genetics*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tyrosine Decarboxylase / genetics*
  • Tyrosine Decarboxylase / isolation & purification*

Substances

  • Tyrosine Decarboxylase