A broad host range plasmid vector that does not encode replication proteins

Eza Kalyaeva; Irina Bass; Gennady Kholodii; Vadim Nikiforov

doi:10.1111/j.1574-6968.2002.tb11208.x

A broad host range plasmid vector that does not encode replication proteins

FEMS Microbiol Lett. 2002 May 21;211(1):91-5. doi: 10.1111/j.1574-6968.2002.tb11208.x.

Authors

Eza Kalyaeva¹, Irina Bass, Gennady Kholodii, Vadim Nikiforov

Affiliation

¹ Institute of Molecular Genetics, Russian Academy of Sciences, 123182, Moscow, Russia.

PMID: 12052556
DOI: 10.1111/j.1574-6968.2002.tb11208.x

Abstract

The 640-bp minimal replication region derived from a plasmid DNA preparation from an Acidothiobacillus ferrooxidans strain capable of autonomous replication in a range of Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Acinetobacter calcoaceticus and Alcaligenes faecalis) was identified. This DNA fragment (named TFK replicon) does not encode Rep proteins and appears to be unrelated to other known replicons.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics*
Base Sequence
DNA Replication / genetics*
DNA, Bacterial / genetics*
Genetic Vectors / genetics
Gram-Negative Bacteria / genetics*
Molecular Sequence Data
Plasmids / genetics*
Replicon / genetics

Substances

Bacterial Proteins
DNA, Bacterial

Associated data

GENBANK/AJ251742