The translation state of differentially expressed mRNAs in the hibernating 13-lined ground squirrel (Spermophilus tridecemlineatus)

Arch Biochem Biophys. 2002 May 15;401(2):244-54. doi: 10.1016/S0003-9861(02)00048-6.

Abstract

The translation state of differentially expressed mRNAs were compared in kidney and brown adipose tissue of the hibernating ground squirrel, Spermophilus tridecemlineatus. Polysome analysis revealed a striking disaggregation of polyribosomes during hibernation and the redistribution of Cox4 (cytochrome c oxidase subunit 4) and Oct2 (organic cation transporter type 2) transcripts into monosome and mRNP fractions of kidney cytoplasmic extracts. Additionally, OCT2 protein levels decreased in kidney of hibernating animals in line with a strong decrease (85%) in translation rate compared with euthermic kidney. There was no translational depression in brown adipose tissue during hibernation and the H isoform of fatty-acid-binding protein (H-FABP), that is up-regulated during hibernation, was increasingly abundant in the heavy polyribosome fraction isolated from the brown adipose of hibernators. This may indicate the existence of a tissue-specific mechanism for the translational control of a subset of genes that are physiologically relevant to the survival of hibernation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue, Brown / metabolism
  • Animals
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Electron Transport Complex IV / genetics
  • Electron Transport Complex IV / metabolism
  • Fatty Acid-Binding Protein 7
  • Fatty Acid-Binding Proteins
  • Gene Expression
  • Hibernation / genetics*
  • Hibernation / physiology
  • In Vitro Techniques
  • Kidney / metabolism
  • Neoplasm Proteins*
  • Nerve Tissue Proteins*
  • Organic Cation Transport Proteins / genetics
  • Organic Cation Transport Proteins / metabolism
  • Organic Cation Transporter 2
  • Polyribosomes / metabolism
  • Protein Biosynthesis
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism
  • Sciuridae / genetics*
  • Sciuridae / physiology
  • Tissue Distribution

Substances

  • Carrier Proteins
  • Fabp7 protein, rat
  • Fatty Acid-Binding Protein 7
  • Fatty Acid-Binding Proteins
  • Neoplasm Proteins
  • Nerve Tissue Proteins
  • Organic Cation Transport Proteins
  • Organic Cation Transporter 2
  • RNA, Messenger
  • SLC22A2 protein, human
  • Slc22a2 protein, mouse
  • Electron Transport Complex IV