Probing the Specificity of a Trypanosomal Aromatic Alpha-Hydroxy Acid Dehydrogenase by Site-Directed Mutagenesis

Biochem Biophys Res Commun. 2002 Apr 26;293(1):633-9. doi: 10.1016/S0006-291X(02)00270-X.

Abstract

The aromatic l-alpha-hydroxy acid dehydrogenase (AHDAH) from Trypanosoma cruzi has over 50% sequence identity with cytosolic malate dehydrogenases (cMDHs), yet it is unable to reduce oxaloacetate. Molecular modeling of the three-dimensional structure of AHADH using the pig cMDH as template directed the construction of several mutants. AHADH shares with MDHs the essential catalytic residues H195 and R171 (using Eventoff's numbering). The AHADH A102R mutant became able to reduce oxaloacetate, while remaining fully active towards aromatic alpha-oxoacids. The Y237G mutant diminished its affinity for all of the natural substrates, whereas the double mutant A102R/Y237G was more active than Y237G and had similar activity with oxaloacetate and with aromatic substrates. The present results reinforce our proposal that AHADH arose by a moderate number of point mutations from a cMDH no longer present in the parasite.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alcohol Oxidoreductases / chemistry
  • Alcohol Oxidoreductases / metabolism*
  • Amino Acid Sequence
  • Amino Acid Substitution
  • Animals
  • Molecular Sequence Data
  • Protein Conformation
  • Protozoan Proteins / chemistry
  • Protozoan Proteins / metabolism*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Substrate Specificity
  • Swine
  • Trypanosoma cruzi / enzymology*

Substances

  • Protozoan Proteins
  • Recombinant Proteins
  • Alcohol Oxidoreductases
  • ahadh1 protein, Trypanosoma cruzi