Myosin X is a downstream effector of PI(3)K during phagocytosis

Nat Cell Biol. 2002 Jul;4(7):469-77. doi: 10.1038/ncb805.


Phagocytosis is a phosphatidylinositol-3-OH-kinase (PI(3)K)-dependent process in macrophages. We identified Myo10 (Myosin-X), an unconventional myosin with pleckstrin homology (PH) domains, as a potential downstream target of PI(3)K. Myo10 was recruited to phagocytic cups in a wortmannin-sensitive manner. Expression of a truncation construct of Myo10 (Myo10 tail) in a macrophage cell line or cytosolic loading of anti-Myo10 antibodies in bovine alveolar macrophages inhibited phagocytosis. In contrast, expression of a Myo10 tail construct containing a point mutation in one of its PH domains failed to inhibit phagocytosis. Expression of Myo10 tail inhibited spreading, but not adhesion, on IgG-coated substrates, consistent with a function for Myo10 in pseudopod extension. We propose that Myo10 provides a molecular link between PI(3)K and pseudopod extension during phagocytosis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Androstadienes / pharmacology
  • Animals
  • Cattle
  • Cell Adhesion
  • Cell Line
  • Macrophages / immunology*
  • Mice
  • Microscopy, Fluorescence
  • Myosins / genetics
  • Myosins / physiology*
  • Phagocytosis
  • Phosphatidylinositol 3-Kinases / metabolism*
  • Point Mutation
  • Pseudopodia / physiology
  • Structure-Activity Relationship
  • Wortmannin


  • Androstadienes
  • Myo10 protein, mouse
  • Phosphatidylinositol 3-Kinases
  • Myosins
  • Wortmannin