Fluorescence in situ hybridization (FISH)--application in research and diagnostics

Folia Histochem Cytobiol. 2002;40(2):107-8.

Abstract

The methods of molecular cytogenetics, in particular fluorescence in situ hybridization (FISH), are widely applied in cytogenetics for identification of numerical and structural chromosomal abnormalities, which are difficult to detect by routine cytogenetic techniques. Due to many advantages, FISH is used in research (gene mapping, gene expression studies, interspecies chromosome homology), and clinical diagnostics (chromosomal aberrations analysis in pre- and postnatal diagnostics, oncology). The techniques of in situ hybridization (ISH) are often employed in addition to classical banding techniques, in case where banding pattern is not reliable. This paper focuses on particular clinical examples, where FISH was successfully used to identify structural and numerical chromosomal aberrations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abortion, Spontaneous / pathology
  • Bone Marrow Cells / drug effects
  • Bone Marrow Cells / metabolism
  • Bone Marrow Cells / ultrastructure
  • Charcot-Marie-Tooth Disease / pathology
  • Chorionic Villi / metabolism
  • Chromosome Painting
  • Chromosomes / drug effects
  • Chromosomes / ultrastructure
  • Chromosomes, Human, Pair 16
  • Genetic Markers
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Lymphocytes / drug effects
  • Lymphocytes / metabolism
  • Lymphocytes / ultrastructure
  • Philadelphia Chromosome
  • Translocation, Genetic
  • Trisomy / pathology

Substances

  • Genetic Markers