Tenascins Are Associated With Lipid Rafts Isolated From Mouse Brain

Biochem Biophys Res Commun. 2002 Jun 14;294(3):742-7. doi: 10.1016/S0006-291X(02)00520-X.


Lipid rafts are microdomains of the plasma membrane which are enriched in glycosphingolipids and specific proteins. The reported interactions of several raft-associated proteins (such as, e.g., F3) with tenascin C and tenascin R prompted us to consider that these oligomeric multidomain glycoproteins of the extracellular matrix (ECM) could associate with rafts. Here, we show punctate immunocytochemical distributions of tenascin C (TN-C) and tenascin R (TN-R) at the membrane surface of neural cells resembling the pattern reported for raft-associated proteins. Moreover, cholesterol depletion with methyl-beta-cyclodextrin reduced the punctate surface staining of TN-C. Consistently, TN-C was associated with lipid rafts of neonatal mouse brain according to sucrose density gradient centrifugation experiments. Furthermore, TN-R was also found in rafts prepared from myelin of adult mice. Thus, brain-derived tenascins are able to associate with lipid rafts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Chemistry
  • Cell Adhesion Molecules / metabolism
  • Cells, Cultured
  • Female
  • Membrane Microdomains / metabolism*
  • Mice
  • Neurons / metabolism*
  • Neurons / ultrastructure
  • Pregnancy
  • Tenascin / metabolism*


  • Cell Adhesion Molecules
  • Tenascin
  • tenascin R