Deoxysarpagine hydroxylase--a novel enzyme closing a short side pathway of alkaloid biosynthesis in Rauvolfia

Bioorg Med Chem. 2002 Aug;10(8):2479-83. doi: 10.1016/s0968-0896(02)00126-8.

Abstract

Microsomal preparations from cell suspension cultures of the Indian plant Rauvolfia serpentina catalyze the hydroxylation of deoxysarpagine under formation of sarpagine. The newly discovered enzyme is dependent on NADPH and oxygen. It can be inhibited by typical cytochrome P450 inhibitors such as cytochrome c, ketoconazole, metyrapone, tetcyclacis and carbon monoxide. The CO-effect is reversible with light (450 nm). The data indicate that deoxysarpagine hydroxylase is a novel cytochrome P450-dependent monooxygenase. A pH optimum of 8.0 and a temperature optimum of 35 degrees C were determined. K(m) values were 25 microM for NADPH and 7.4 microM for deoxysarpagine. Deoxysarpagine hydroxylase activity was stable in presence of 20% sucrose at -25 degrees C for >3 months. The analysis of presence of the hydroxylase in nine cell cultures of seven different families indicates a very limited taxonomic distribution of this enzyme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aryl Hydrocarbon Hydroxylases / antagonists & inhibitors
  • Aryl Hydrocarbon Hydroxylases / metabolism*
  • Aryl Hydrocarbon Hydroxylases / radiation effects
  • Carbon Monoxide / pharmacology
  • Cytochrome P-450 Enzyme System
  • Hydrogen-Ion Concentration
  • Indole Alkaloids / metabolism
  • Kinetics
  • Light
  • NADP
  • Plant Proteins / antagonists & inhibitors
  • Plant Proteins / metabolism
  • Plant Proteins / radiation effects
  • Rauwolfia / enzymology*
  • Secologanin Tryptamine Alkaloids / biosynthesis*
  • Temperature

Substances

  • Indole Alkaloids
  • Plant Proteins
  • Secologanin Tryptamine Alkaloids
  • NADP
  • Carbon Monoxide
  • Cytochrome P-450 Enzyme System
  • Aryl Hydrocarbon Hydroxylases
  • deoxysarpagine hydroxylase
  • sarpagine