Studies on the Anti-Inflammatory Activity of Phytopharmaceuticals Prepared From Arnica Flowers

Planta Med. 2002 May;68(5):385-91. doi: 10.1055/s-2002-32067.

Abstract

Phytopharmaceuticals prepared from flowerheads of Arnica montana of Spanish origin and of the new type "Arbo", which can be easily and economically cultivated, were studied for their capability to impair activation of the transcription factors NF-kappa B and NF-AT. Both proteins are responsible for the transcription of genes encoding various inflammatory mediators. Additionally, their influence on the release of the cytokines IL-1 and TNF-alpha were examined. The inhibitory activities correlate with their quantitative and qualitative content of sesquiterpene lactones (Sls). Moreover, it was shown that the inhibitory potency of 11 alpha,13-dihydrohelenalin derivatives being the main Sls in the Spanish flowers depend on their esterfication. Compounds with unsaturated acyl moieties, such as methacrylate and tiglinate, exhibited a stronger activity in the NF-kappa B EMSA as well as in the croton oil ear test in mice than the acetate derivative.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Arnica*
  • Cell Survival / drug effects
  • Croton Oil / pharmacology
  • DNA-Binding Proteins / drug effects
  • DNA-Binding Proteins / metabolism
  • Dose-Response Relationship, Drug
  • Guinea Pigs
  • Humans
  • Indomethacin / pharmacology
  • Interleukin-1 / metabolism
  • Jurkat Cells / drug effects
  • Lactones / isolation & purification
  • Lactones / pharmacology
  • Leukocytes, Mononuclear / cytology
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / metabolism
  • Male
  • Mice
  • Molecular Structure
  • NF-kappa B / drug effects
  • NF-kappa B / metabolism
  • NFATC Transcription Factors
  • Nuclear Proteins*
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Plant Stems / chemistry
  • Sesquiterpenes / chemistry
  • Sesquiterpenes / isolation & purification
  • Sesquiterpenes / pharmacology
  • Transcription Factors / drug effects
  • Transcription Factors / metabolism
  • Tumor Necrosis Factor-alpha / drug effects
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Anti-Inflammatory Agents
  • DNA-Binding Proteins
  • Interleukin-1
  • Lactones
  • NF-kappa B
  • NFATC Transcription Factors
  • Nuclear Proteins
  • Plant Extracts
  • Sesquiterpenes
  • Transcription Factors
  • Tumor Necrosis Factor-alpha
  • plenolin
  • Croton Oil
  • Indomethacin