Effect of the essential oil from the flowers of Magnolia sieboldii on the lipopolysaccharide-induced production of nitric oxide and prostaglandin E2 by rat peritoneal macrophages

Planta Med. 2002 May;68(5):459-62. doi: 10.1055/s-2002-32085.

Abstract

The essential oil from the flowers of Magnolia sieboldii was tested for its effects on lipopolysaccharide (LPS)-induced production of nitric oxide (NO) and prostaglandin E(2) (PGE(2)) by rat peritoneal macrophages. It was shown to induce the production of NO and PGE 2 in a concentration-dependent manner (3 - 30 microg/ml). Gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) led to the identification of sixty compounds, of which beta-elemene (18.0 %), alpha-terpinene (14.83 %) and beta-myrcene (12.72 %) were the major constituents. Among these three compounds, alpha-terpinene was found to be the most effective one with inhibitory activity on NO and PGE(2) production by LPS-stimulated rat peritoneal macrophages.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyclic Monoterpenes
  • Animals
  • Cell Survival / drug effects
  • Cyclohexane Monoterpenes
  • Dinoprostone / biosynthesis*
  • Dose-Response Relationship, Drug
  • Lipopolysaccharides / pharmacology*
  • Macrophages, Peritoneal / cytology
  • Macrophages, Peritoneal / drug effects
  • Macrophages, Peritoneal / metabolism
  • Magnoliaceae*
  • Mitochondria / drug effects
  • Mitochondria / metabolism
  • Monoterpenes*
  • Nitric Oxide / biosynthesis*
  • Nitrites / metabolism
  • Oils, Volatile / isolation & purification
  • Oils, Volatile / pharmacology*
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology
  • Plant Stems / chemistry
  • Rats
  • Sesquiterpenes*
  • Terpenes / isolation & purification
  • Terpenes / pharmacology

Substances

  • Acyclic Monoterpenes
  • Cyclohexane Monoterpenes
  • Lipopolysaccharides
  • Monoterpenes
  • Nitrites
  • Oils, Volatile
  • Plant Extracts
  • Sesquiterpenes
  • Terpenes
  • beta-elemene
  • beta-myrcene
  • Nitric Oxide
  • gamma-terpinene
  • Dinoprostone