A piperidine amide extracted from Piper longum L. fruit shows activity against Aedes aegypti mosquito larvae

J Agric Food Chem. 2002 Jun 19;50(13):3765-7. doi: 10.1021/jf011708f.


Mosquito larvicidal activity of Piper longum fruit-derived materials against the fourth-instar larvae of Aedes aegypti was examined. A crude methanol extract of P. longum fruits was found to be active against the larvae, and the hexane fraction of the methanol extract showed a strong larvicidal activity of 100% mortality. The biologically active component of P. longum fruits was characterized as pipernonaline by spectroscopic analyses. The LC(50) value of pipernonaline was 0.25 mg/L. The toxicity of pipernonaline is comparable to that of pirimiphos-methyl as a mosquito larvicide. In tests with available components derived from P. longum, no activity was observed with piperettine, piperine, or piperlongumine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aedes* / growth & development
  • Alkaloids / chemistry
  • Alkaloids / isolation & purification*
  • Animals
  • Fruit / chemistry*
  • Insecticides / chemistry
  • Insecticides / isolation & purification*
  • Larva
  • Methanol
  • Piperaceae / chemistry*
  • Piperidines*
  • Plant Extracts / chemistry*


  • Alkaloids
  • Insecticides
  • Piperidines
  • Plant Extracts
  • pipernonaline
  • Methanol