Constitutive expression of ETHYLENE-RESPONSE-FACTOR1 in Arabidopsis confers resistance to several necrotrophic fungi

Plant J. 2002 Jan;29(1):23-32. doi: 10.1046/j.1365-313x.2002.01191.x.


Infection of a plant by a pathogen induces a variety of defense responses that imply the action of several signaling molecules, including salicylic acid (SA), jasmonic acid (JA) and ethylene (E). Here we describe the role of ETHYLENE-RESPONSE-FACTOR1 (ERF1) as a regulator of ethylene responses after pathogen attack in Arabidopsis. The ERF1 transcript is induced on infection by Botrytis cinerea, and overexpression of ERF1 in Arabidopsis is sufficient to confer resistance to necrotrophic fungi such as B. cinerea and Plectosphaerella cucumerina. A positive co-operation between E and SA pathways was observed in the plant response to P. cucumerina. Infection by Pseudomonas syringae tomato DC3000, however, does not affect ERF1 expression, and activation of ethylene responses by ERF1 overexpression in Arabidopsis plants reduces tolerance against this pathogen, suggesting negative crosstalk between E and SA signaling pathways, and demonstrating that positive and negative interactions between both pathways can be established depending on the type of pathogen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / drug effects
  • Arabidopsis / genetics*
  • Arabidopsis / microbiology
  • Arabidopsis Proteins / genetics*
  • Botrytis / growth & development
  • Botrytis / pathogenicity
  • Cyclopentanes / pharmacology
  • DNA-Binding Proteins
  • Ethylenes / pharmacology
  • Gene Expression Regulation, Plant / drug effects
  • Immunity, Innate / drug effects
  • Mitosporic Fungi / growth & development*
  • Mitosporic Fungi / pathogenicity
  • Nuclear Proteins / genetics*
  • Oxylipins
  • Plant Diseases / genetics
  • Plant Diseases / microbiology*
  • Plant Growth Regulators / pharmacology*
  • Plant Proteins
  • Pseudomonas / growth & development
  • Pseudomonas / pathogenicity
  • Salicylic Acid / pharmacology
  • Signal Transduction / drug effects
  • Transcription Factors


  • Arabidopsis Proteins
  • Cyclopentanes
  • DNA-Binding Proteins
  • Ethylenes
  • Nuclear Proteins
  • Oxylipins
  • Plant Growth Regulators
  • Plant Proteins
  • Transcription Factors
  • ethylene-responsive element binding protein
  • jasmonic acid
  • ethylene
  • Salicylic Acid